Packaging and storage
Preserve in tight containers.
Labeling
Label it to indicate that it is intended for veterinary use only.
Identification
Shake a suitable quantity of Powder with methanol to obtain a solution containing the equivalent of 1 mg of tetracycline hydrochloride per mL, and filter. Using the filtrate so obtained as the
Test Solution, proceed as directed under
IdentificationTetracyclines
193
.
Loss on drying
731
Dry about 100 mg, accurately weighed, in a capillary-stoppered bottle in vacuum at a pressure not exceeding 5 mm of mercury at 60

for 3 hours: it loses not more than 2.0% of its weight.
Assay
Transfer an accurately weighed quantity of Powder to a high-speed blender jar containing an accurately measured volume of 0.1 N hydrochloric acid, so that the solution so obtained contains not less than 150 µg of tetracycline hydrochloride per mL, and blend for about 3 to 5 minutes. Proceed as directed for tetracycline under
AntibioticsMicrobial Assays
81
, diluting an accurately measured volume of this solution quantitatively and stepwise with water to yield a
Test Dilution having a concentration assumed to be equal to the median dose level of the Standard.